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. Author manuscript; available in PMC: 2008 Aug 14.
Published in final edited form as: J Immunol. 2007 Nov 15;179(10):6836–6844. doi: 10.4049/jimmunol.179.10.6836

Figure 4. In vitro suppression assays.

Figure 4

(A) Thymidine incorporation assays were used to assess in vitro suppressive function at a range of effector:Treg ratios. Thymic CD4+CD25- effectors and Tregs were used. +/+, wild-type; -/-, SAP-1 deficient, white bars, wild-type; black bars, SAP-1-/-. CFSE suppression assays performed at a 1:1 ratio of Teffector:Treg. Grey shaded area, activated effectors at 72hrs; dark grey line, 1:1 ratio of Teffectors:Tregs; light grey line, unstimulated effectors. Top panel wild-type thymocytes, Bottom panel SAP-1-/- thymocytes (B) Suppression assay, assessed by thymidine incoportaion after 72hrs. White bars, wild-type; black bars, DN Raf T cells. CD4+CD25- effectors and Tregs were taken from lymph nodes.