Figure 1.

Schematic representation of tagged Act88F transformation constructs. The endogenous Act88F gene inserted into the pW8 vector (A) was modified at the C terminus by PCR and site-directed mutagenesis to contain a sequence corresponding to six consecutive histidines (B) or to an 11-mer derived from vesicular stomatitis virus G protein (C). This alteration resulted in the removal of the 3′ UTR. In the corresponding control construct, the 3′ UTR was removed from the endogenous Act88F (D). For tagging the Act88F at the N terminus, the sequences corresponding to six histidines or the 11-mer were introduced in the Act88F gene following the translation start site (E and F). Act88F coding sequences are represented by black boxes, whereas noncoding sequences are shown as white boxes. The epitope tags are shown as shaded boxes. Elements of the pW8 transformation vector are represented by stippled boxes.