Figure 2.

Expression of epitope-tagged Act88F in transformed KM88 flies. Protein extracts corresponding to an equal number of thoraces from transformants established with C-terminally (A) and N-terminally (B) tagged Act88F constructs were analyzed by immunoblotting. Wild-type (WT) and KM88 protein extracts were included on each blot as controls. Immunblots were incubated with either a mAb recognizing the 6xHis tag (left) or the 11-mer tag (right). (A) Each antibody recognizes a single band in extracts prepared from the transformed lines, which is absent in thoracic extracts from wild-type and KM88 flies. Its apparent molecular mass of 43 kDa corresponds to that of actin. Levels of tagged Act88F protein do not vary significantly among the transformants expressing the same construct. (B) In immunoblots from N-terminally tagged Act88F transformants, an additional band migrating at 55 kDa is detected. This band corresponds to tagged arthrin. In protein extracts from transformants tagged with the 11-mer at the N terminus, multiple actin and arthrin bands are present.