Figure 3.

Effects of epitope tagging on IFM function in transformed Drosophila. Flight ability was examined in a flight tester (Okamoto et al., 1986). Accordingly, the “landing sites” of individual flies were scored and are shown as percentages. A consistent fraction of flies (approximately one-third) was found either at the bottom of the cylinder or remained in the saucer and was subtracted from the nonflying flies from each test. Each histogram represents the average of three experiments. (A) Wild-type flies show heterogeneity in their flight ability. (B) The KM88 null mutant is flightless. (C) In N-terminally tagged 6xHis transformants, the percentage of poor fliers is higher than in wild-type flies. (D) C-terminally tagged 6xHis transformants are not able to fly. (E) Transformants with the 11-mer tag at the N terminus have a slightly increased flight ability compared with flies with a 6xHis tag, but they do not fly as proficiently as wild-type flies. (F) Similar to the C-terminally tagged 6xHis transformants, C-terminally tagged 11-mer transformants are flightless.