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. Author manuscript; available in PMC: 2009 Jul 1.
Published in final edited form as: Mol Genet Metab. 2008 May 23;94(3):326–335. doi: 10.1016/j.ymgme.2008.03.013

Figure 6.

Figure 6

BHMT and BHMT2 bacterial expression. (A) BHMT and BHMT2 were expressed as GST fusion proteins in BL21 E. coli. The GST-tags were removed by PreScission Protease after protein purification. (B) Recombinant BHMT and BHMT2 enzyme activity. Equal quantities of bacterially expressed BHMT and BHMT2 were used to perform enzyme assays with L-homocysteine and betaine as substrates. (C) Size-exclusion chromatography of purified bacterially expressed recombinant GST-BHMT and GST-BHMT2 proteins. Protein profiles are expressed as percentages of peak milliabsorbance units (mAu). The initial peaks for both preparations eluted with the void volume.