TABLE 2.

Repair of HO-induced DSBs in strain AM1003 and its derivatives

			% phenotype of colonies
Relevant genotype	Strain	No. of colonies tested	Ade+ Leu+ (GC)	Ade+ Leu+/− (GC/BIR)	Ade+ Leu− (BIR)a	Ade+/−r Leu− (BIR/loss)a	Ade−r Leu− (loss)	Ade−w Leu− (HCO)	Partial Ade−w Leu− (partial HCO)b	Viability YEP-Gal (%)
Wt	AM1003	671	7.5	2.1	78.1	7.4	1.5	0.3	3.1	94 ± 11
pol32Δ	AM1014, AM1152c	1549	0.5	0.0	18.9*	19.0	38.0	7.0	16.6	90 ± 11
ard1Δ	AM1108	539	6.9	7.2	72.7	7.8	2.4	1.1	1.9	96 ± 10
rad51Δ	AM 1079	134	0.8	0.0	0.8*	31.3	67.1	0.0	0.0	86 ± 18
rtt107Δ	AM1089	351	7.4	0.0	72.6	12.3	6.0	0.0	1.7	79 ± 15
sgs1Δ	AM1029	375	19.5	1.3	66.8*	5.6	2.1	1.3	3.4	95 ± 13
mms1Δ	AM1024	356	4.5	0.0	72.8	14.0	4.2	0.6	3.9	88 ± 11
tof1Δ	AM1030	318	2.5	0.0	81.4	6.9	3.8	0.0	5.4	94 ± 16
rtt101Δ	AM1028	393	10.4	0.8	66.2*	17.0	3.8	1.0	0.8	91 ± 5
elg1Δ	AM1021	336	5.7	6.3	72.0	11.3	0.9	1.2	2.6	80 ± 12
mms2Δ	AM1025	326	8.0	1.2	69.9	14.8	1.2	0.6	4.3	98 ± 11
rad5Δ	AM1027	372	2.2	0.0	83.9	10.8	0.0	0.0	3.1	86 ± 9
mms22Δ	AM1099	259	7.0	0.3	79.5	7.0	4.3	0.4	1.5	84 ± 10
cac2Δ	AM1018	307	6.5	0.3	77.6	12.4	0.0	0.0	3.2	99 ± 9

Ade^−r, Ade⁻His⁺ red colonies indicative of the ade1ADE3 genotype; Ade^−w, Ade⁻His⁻ white colonies indicative of the ADE1ade3 genotype; GC, gene conversion; BIR, break-induced replication; HCO, half-crossover; wt, wild type. Sectored colonies are indicated by both phenotypes separated by a slash (/). *A statistically significant difference from the isogenic wild-type strain (AM1003; P < 0.05).

^aAt least some of these events could be half-crossovers (primarily in pol32Δ, see the text for details) or GCRs (primarily in rad51Δ, but also in other strains, see the text for details).

^bPartial HCOs in which part of the colony was Ade^−w while the other part represented chromosome loss or BIR.

^cData obtained for two isogenic pol32Δ strains [AM1152, which contains the URA3 marker inserted 3 kb proximal to MATa (see Figure 1F), and AM1014 (see Figure 1A)] were indistinguishable from each other and therefore combined.