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. 2008 Aug;179(4):1919–1932. doi: 10.1534/genetics.108.089318

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Copyright © 2008 by the Genetics Society of America

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Figure 4.— — DPI disrupts ROS gradient and induces hyphal morphology defects. (A) Light micrographs showing localization of ROS during conidial germination. Germlings of A28 wild-type strain grown on MN medium for 12 hr followed by treatment with 1 μm of DPI for 2 hr and stained with NBT. Bars, 10 μm. (B) The percentage of hyphae >25 μm containing an ROS gradient at the tip in untreated controls or following treatment with 1 or 5 μm of DPI for 2 hr. Bars show the average of three independent experiments where 200 tips were examined in each replicate. (C) Light micrographs of untreated control hyphae or hyphae treated with 1 μm of DPI for 2 hr. Arrows show abnormal hyphal-tip morphologies induced by DPI. (D) The percentage of conidia that germinated in the presence of DPI and ascorbic acid. Germlings of A28 wild-type strain were germinated on YGV medium for 16 hr in the presence of the indicated concentrations of DPI and AAc. Bars show the average of three independent experiments where 200 germlings were examined in each replicate.

Figure 4.— — DPI disrupts ROS gradient and induces hyphal morphology defects. (A) Light micrographs showing localization of ROS during conidial germination. Germlings of A28 wild-type strain grown on MN medium for 12 hr followed by treatment with 1 μm of DPI for 2 hr and stained with NBT. Bars, 10 μm. (B) The percentage of hyphae >25 μm containing an ROS gradient at the tip in untreated controls or following treatment with 1 or 5 μm of DPI for 2 hr. Bars show the average of three independent experiments where 200 tips were examined in each replicate. (C) Light micrographs of untreated control hyphae or hyphae treated with 1 μm of DPI for 2 hr. Arrows show abnormal hyphal-tip morphologies induced by DPI. (D) The percentage of conidia that germinated in the presence of DPI and ascorbic acid. Germlings of A28 wild-type strain were germinated on YGV medium for 16 hr in the presence of the indicated concentrations of DPI and AAc. Bars show the average of three independent experiments where 200 germlings were examined in each replicate.