TABLE 2.
ROS staining pattern of different hyphal morphology mutants
| Strain
|
||||||
|---|---|---|---|---|---|---|
| Class | Wild type | ΔnoxA | ΔnoxR | ΔracA | Δcdc42 | ΔbemA |
| 1 | 6.0 (±2.1) | 2.5 (±0.7) | 1.5 (±0.7) | 2.0 (±0.0) | 11.5 (±3.5) | 3.5 (±0.7) |
| 2 | 7.0 (±1.7) | 2.0 (±0.2) | 2.0 (±1.4) | 7.5 (±0.7) | 2.0 (±0.3) | 4.0 (±0.8) |
| 3 | 6.5 (±2.5) | 19.0 (±2.7) | 7.0 (±0.6) | 5.5 (±0.7) | 15 (±0.7) | 25.0 (±4.2) |
| 4 | 74.5 (±7.5) | 72.5 (±7.7) | 23.0 (±4.2) | 21.5 (±3.5) | 19.0 (±1.4) | 62.5 (±3.5) |
| 5 | 6.0 (±1.4) | 4.0 (±1.4) | 25.5 (±4.9) | 44.0 (±2.8) | 7.0 (±1.4) | 5.0 (±1.2) |
| Abnormal | 0.0 (±0.0) | 0.0 (±0.0) | 41.0 (±5.8)a | 19.5 (±4.5)a | 45.5 (±5.5)b | 0.0 (±0.0) |
Hyphae were grown for 16 hr at 28° on coverslips in YGV media before staining with NBT as described in materials and methods. Numbers are percentages.
a
ROS staining confined to the middle regions of germlings but not at hyphal tip.
b
Germlings >25 μm with uniform distribution of ROS.