TABLE 4.
Wild type | ΔatmA | ΔnoxR | ΔatmA ΔnoxR | |
---|---|---|---|---|
% pol | 80.6 (±7.5) | 22.6 (4.1) | 48.6 (±6.4) | 80.6 (±2.5) |
% pol (n ≥ 4) | 100.0 (±0.0) | 67.3 (±3.0) | 99.6 (±1.5) | 100.0 (±0.0) |
No. of nuclei | ||||
1 | 6.6 (±1.1) | 6.0 (±2.0) | 45.0 (±4.2) | 7.6 (±1.4) |
2 | 32.3 (±4.0) | 13.0 (±1.0) | 41.0 (±4.4) | 24.3 (±3.2) |
4 | 41.0 (±2.6) | 41.3 (±3.1) | 13.0 (±3.7) | 43.0 (±3.6) |
>4 | 20.0 (±5.2) | 41.6 (±3.7) | 1.0 (±0.4) | 25.3 (±6.1) |
Hyphae were examined following 12 hr growth on YGV at 28°. “% pol” refers to the percentage of conidiospores that possess a recognizable germ tube. “% pol (n ≥ 4)” refers to the percentage of conidiospores with four or more nuclei that possess a recognizable germ tube. The number of nuclei was determined by Hoechst 33258 staining as described in materials and methods. Numbers are percentages.