Figure 1.—
Regulation of lin-3 transcription by the synMuv genes. Real-time RT–PCR experiments were performed on the strains shown. Mean ΔΔCT values were used to calculate relative changes in lin-3 expression normalized to levels of rpl-26. Mean values and standard deviations of relative lin-3/rpl-26 ratios for the trials are shown. (A) Mutation of each class A synMuv gene in a class B synMuv mutant background has increased lin-3 transcription. (B) Mutation of each class B synMuv gene in a class A synMuv mutant background has increased lin-3 transcription. lin-15A(n767) and lin-61(n3809) were used for this experiment. (C) Two class B–B–A synMuv triple mutants have increased levels of lin-3 transcription compared to their respective class B–A double mutants. lin-15A(n433) and lin-61(n3809) were used for this experiment.