Table 1.
Concentration of uPAR in clathrin-coated pits of nonpolarized MDCK cells as determined by immunogold labeling of ultracryosections
| Total no. of gold particles counted | % Gold particles in coated pits | Total amount (length) of surface included (μm) | % of surface occupied by coated pitsa | Concentration of uPAR in coated pits (fold) | |
|---|---|---|---|---|---|
| Clone 8.1 (without bound uPA:PAI-1) | 8529 | 0.8 | 705 | ∼1.3 | 0.6 |
| Clone 3.2 (with bound uPA:PAI-1) | 1095 | 1.1 | 730 | ∼1.3 | 0.8 |
a
Calculation of the fractional surface area occupied by coated pits is based on an average of both clones.