Figure 2.

Time dependent changes in the absorption spectrum of dioldehydrase-adenosylcobalamin complex.

(A) Absorption spectra of aerobic dioldehydrase incubated with 10 mM potassium acetate and 13 μM adenosylcobalamin from 2 min to 58 min. (B) Absorbance change at 362 nm for 1) the experiment in panel A, and analogous experiments performed in the absence of 2) O₂ and 3) activating monocations.