Fig. 2.

Electrostatic interactions between VirE1 and VirE2. (A) Residues from both domains of VirE2 form electrostatic interactions (dashed lines) with acidic residues from VirE1 (colors as in Fig. 1A). (B) A representation of the electrostatic surface potential of VirE1 showing an overall negative charge (red). The orientation of VirE1 is as in C [Figure created with PyMol (51)]. (C) A representation of the electrostatic surface potential of VirE2 is shown, calculated in the absence of VirE1. Although the VirE2 surface potential has both positive and negative regions, the VirE1 binding pocket is predominantly electropositive (blue). The single helix in VirE1 is depicted as a yellow diagram in the same orientation as in B.