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Helicase translocation requires appropriate facing of the origin motifs. (A) FI* reactions containing increasing amounts of the DnaBC helicase complex (at 0, 100, 300, 600 ng) on wild-type or mutant templates containing insertions of 6 bp (oriV3–6) or 11 bp (oriV3–11) between the DnaA-box region and the AT-rich region containing 13-mers. (B) Results obtained in EM experiments performed for those mutants as described in Materials and Methods.
