Fig. 4.
Twelve hours of GABAA receptor blockade did not alter the amplitude of GABA or AMPA mPSCs. (A) Graph of the average amount of time chick embryos moved limbs during a 5-min period of observation. Data are normalized to control values at each time point in which observations were collected. Blocking GABAA receptors in ovo (gabazine) for 12 h, beginning at E8 or at E9.5, transiently reduces the embryonic limb movements, which recover within 12 h. (B and C) The average amplitude of GABA mPSCs (B) or AMPA mPSCs (C) is not significantly different among motoneurons from embryos treated with saline (control) or gabazine for 12 h. Gabazine treatment for 48 h significantly increased the amplitude of GABA mPSCs or AMPA mPSCs compared with control (48-h data same as that in Fig. 3 D and E). Dashed lines represent control values. Error bars, SE.