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. 2008 Aug 4;105(32):11242–11247. doi: 10.1073/pnas.0804904105

Fig. 4.

Fig. 4.

Pitx2c regulates PGC survival and proliferation. Asymmetric expression of cyclinD1 is detected at stage 28 HH (A). Expression of cyclinD1 is induced after pitx2c overexpression in the right gonad (B) when compared with a control (A/P-infected) right gonad in A. Overexpression of cyclinD1 induces right gonad outgrowth (C) when compared with a control (Fig. 3E). Histological sections showing symmetric vasa expression at the time when PGCs migrate toward the gonads in control embryos at stage 20 HH (G) and in pitx2c transfected gonads (H). Red and black arrowheads show symmetric PGC numbers in wild-type and pitx2 transfected embryos in G and H, respectively. (D and E) Asymmetric distribution of vasa transcripts at stage 23 HH (D) and stage 29 HH (E). vasa (F) and oct4 (J) transcripts are highly detected in the left gonad at stages 35 and 34 HH, respectively, but almost absent in the regressing right gonad at stage 38 (K and not shown for vasa). After pitx2c overexpression, vasa and oct4 expression is restored in the right gonad and detection of both transcripts is symmetrical (I and L, respectively). Red and black arrowheads points to the right and left gonad, respectively.