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. 2008 Aug 11;105(32):11299–11304. doi: 10.1073/pnas.0801457105

Fig. 3.

Fig. 3.

Secreted proteins of telomere-dysfunctional mice are overexpressed in presenescent human cells and in response DNA damage. BJ fibroblasts were used for the analyses. Under our laboratory conditions, the proliferation of BJ cells slowed down at PD60 (presenescence). The cells were fully senescent at PD70. (A) The histogram shows mRNA expression in BJ cells at the indicated PD (n = 3 repeat experiments). The bars show mean values; error bars show standard deviation. (B) Representative Western blots on the expression of marker proteins in BJ cells at the indicated PD (n = 3 repeat experiments). (C–E) The histograms show protein expression (C and E) and enzyme activity (D) of the indicated markers in culture medium incubated for 4 h on BJ-cells at the indicated PD (n = 3 repeat experiments). The dots show mean values; the error bars show standard deviation. Note that the expression of marker proteins increases in presenescent BJ-cells at PD60. (F) The histogram shows mRNA expression of the indicated biomarkers relative to GAPDH in irradiated (4 h after 4-Gy γ-irradiation) and nonirradiated BJ-cells at the indicated PD (n = 3 repeat experiments). The bars show mean values; error bars show standard deviation. (G) Representative Western blots on the expression of marker proteins in irradiated (4 h after 4-Gy γ-irradiation) and nonirradiated BJ-cells at the indicated PD (n = 3 repeat experiments). (H) The histograms show protein expression (CRAMP, stathmin, EF-1α) and enzyme activity (chitinase) in culture medium incubated for 4 h on irradiated (4-Gy γ-irradiation) and nonirradiated BJ-cells at the indicated PD.