Table 3.
Summary of immunoelectron microscopic colocalization of Br-RNA and of transcription and processing factors
| Nuclear area | Antibody | 4–5 min | 6–7 min | 8–9 min | 10–11 min | 12–13 min | 14–15 min | 20–25 min | 30–35 min | 90 min |
|---|---|---|---|---|---|---|---|---|---|---|
| PF | Anti-pol II | xxx | xxx | xxx | xx | xx | xx | xx | xx | xx |
| Anti-hnRNP | x | x | xx | xx | xx | xx | xxx | xxx | − | |
| Anti-SC35 | x | xx | xx | xx | xx | xx | xx | xx | − | |
| Anti-Sm complex | x | xx | xx | xx | xx | xx | xx | xxx | − | |
| Anti-poly(A)pol | x | x | x | x | x | x | x | x | − | |
| Anti-TFIIH | xx | xx | xx | xx | xx | xx | xx | xx | − | |
| IG | Anti-pol II | o | o | o | o | o | o | o | o | − |
| Anti-hnRNP | o | o | o | o | o | o | o | o | − | |
| Anti-SC35 | o | o | o | o | o | x | x | xx | − | |
| Anti-Sm complex | o | o | o | o | o | x | x | xx | − | |
| Anti-poly(A)pol | o | o | o | o | o | o | o | o | − | |
| Anti-TFIIH | o | o | o | o | o | x | xx | xx | − | |
| IGAZ | Anti-pol II | o | o | o | o | x | x | x | x | − |
| Anti-hnRNP | o | o | o | o | o | o | o | o | − | |
| Anti-SC35 | o | o | o | o | o | o | o | o | − | |
| Anti-Sm complex | o | o | o | o | x | x | x | xx | − | |
| Anti-poly(A)pol | o | o | o | o | o | o | o | o | − | |
| Anti-TFIIH | o | o | o | o | x | x | x | x | − |
o, x, xx, xxx, Estimation of intensity of colocalization with anti-BrdU antibodies; PF, perichromatin fibrils; IG, interchromatin granules; IGAZ, IG-associated zones; −, not done.