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. Author manuscript; available in PMC: 2009 Jun 1.
Published in final edited form as: Methods. 2008 May 29;45(2):172–181. doi: 10.1016/j.ymeth.2008.03.004

Figure 3. Expression of eGFP driven by the Gdf9 promoter in cultured ES cells.

Figure 3

(A, B) eGFP-positive cells seen at d1 of in vitro differentiation of JM21 XX ES cells. Structures resembling polar bodies (black arrow) and a zona pellucida (red arrow) are seen. eGFP fluorescence represented in A, Hoechst DNA stain in B. (C) Hoechst stain of an oocyte-like cell at d1 of differentiation in metaphase stage of mitosis as determined by chromosomal alignment along a metaphase plate (white arrow). (D) eGFP positive zygote-like structure seen at d2 of in vitro differentiation and (E) Four-cell Hoechst stained cell seen at d2 of in vitro differentiation. (A-E, a): Phase images. Images in A and D = 20X magnification, Images in B, C and E = 40X magnification. Scale bars = 20μm (A, D), 25μm (B, C, E).