Fig. 2.

Adenovirus packaging of vectors capable of producing short hairpin RNA-targeting CIAPIN1 was constructed and proved to be specific and potent in silencing CIAPIN1 expression in four HCC cell lines. (A) Indicated cells were infected with AdSiGFP (control) or AdSiCIAPIN1. Cell lysates were harvested at 48 or 96 h after infection and detected by anti-CIAPIN1 and anti-β-actin antibodies. (B) Real-time reverse transcription–PCR of CIAPIN1 mRNA from SMMC-7721 cells harvested 2 days after infection at the indicated MOIs showed a dose-dependent decrease in mRNA levels. At an MOI of 30, the AdSiCIAPIN1 suppresses the expression of CIAPIN1 mRNA by up to 80% compared with the mock group.