Fig. 5.

Local injection of adenovirus-delivered CIAPIN1 siRNA suppresses the established tumor growth in nude mice. (A) Two weeks after subcutaneous inoculation of SMMC-7721 cells, three groups of nude mice were treated every other day for 10 days by intratumor injection of AdSiCIAPIN1 or AdSiGFP at 2.0 × 10⁸ plaque-forming units per animal or mock as a control (arrow). Representative photographs of a mouse in each group 6 weeks later are shown. (B) Quantitation of tumor volume shown in (A). The tumor size was measured and the tumor volume was calculated weekly. Data are presented as mean ± standard deviation (n = 6). Error bars: standard deviation. (C) In situ terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling apoptosis analysis of tumor sections derived from mice receiving different treatments. The apoptotic nuclei were stained dark brown (original magnification ×200).