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Heterologous competition binding curves with buprenorphine as competitor for μ, δ, and κ receptor sites. [3H]DAMGE (1 nM) binding was determined in P1 (upper) and adult (lower) brain membranes in the presence of 12–16 concentrations of buprenorphine. [3H]DPDPE (2 nM) and [3H]U69593 (1 nM) binding was conducted with adult brain membranes. Corresponding Bmax and Ki values are given in Table 1. Results are from three to nine separate experiments.
