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Disruption of dymA by gene replacement. (A) Known map of wild-type dymA locus. (B) Map of dymA− cells as derived from the Southern blot shown in C. The lighter lines with numbers underneath indicate the size of the restriction fragment (in kilobases) hybridizing with the probe when genomic DNA is digested with the enzymes shown on the right (X, XbaI; C, ClaI; N, NdeI; B, BamHI; E, EcoRV; S, SpeI; RI, EcoRI).
