Figure 6.

Cells lacking dynamin A are large and irregularly shaped and show multiple pseudopodia. (A) Phase-contrast micrograph of untransformed Ax2 cells in axenic culture. (B) Phase-contrast micrograph of Ax2 cells 4 h after transfer in a nutrient-free, buffered salt solution. (C and D) Dynamin A-depleted cells show a striking difference in size and morphology and appear apolar in axenic culture. (E and F) When placed under starvation conditions for 4 to 5 h, dymA⁻ cells take on a very elongated shape, form many cell surface projections, and show increased motile activity. Bar, 10 μm.