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. Author manuscript; available in PMC: 2008 Aug 15.
Published in final edited form as: Chem Res Toxicol. 2006 Oct;19(10):1366–1373. doi: 10.1021/tx060106t

Fig. 2.

Fig. 2

Velocity versus substrate concentrations for reduction of NHOH-4-ABP by (A) pooled human liver microsomes (250 μg), and (B) purified human recombinant b5R and cyt b5 (1:10 stoichiometry). All reactions were in PBS, pH 7.4, with 1 mM NADH and 3 mM ascorbate for arylhydroxylamine stabilization. Data for both enzyme sources were each fit to a one-site Michaelis Menten equation.