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. Author manuscript; available in PMC: 2008 Aug 15.
Published in final edited form as: Chem Res Toxicol. 2006 Oct;19(10):1366–1373. doi: 10.1021/tx060106t

Fig. 5.

Fig. 5

(A) Microsomal content of NADH cytochrome b5 reductase (as measured by immunoreactivity to b5R antibodies) did not correlate with NHOH-4-ABP reduction activity (500 μM substrate) in 11 individual human liver microsomes (r = −0.11). However, there was only a 1.8-fold range in b5R content in this sample of microsomes. (B) Microsomal cyt b5 content (as measured by immunoreactivity to anti-cyt b5 antibody) did correlate significantly with NHOH-4-ABP reduction in 11 individual human liver microsomes (r = 0.79; P = 0.004). Activity results are representative of three experiments each performed in duplicate. Reactions included human liver microsomes (250 μg), NHOH-4-ABP (500 μM), 1 mM NADH, and ascorbate (3 mM), in PBS, pH 7.4.