. Author manuscript; available in PMC: 2008 Aug 15.

Published in final edited form as: Chem Res Toxicol. 2006 Oct;19(10):1366–1373. doi: 10.1021/tx060106t

Table 3.

Carcinogenic arylhydroxylamine reduction by microsomes and cytosol prepared from pooled human breast, liver, and colon tissues.^a

	NHOH-4-ABP reduction (nmol/mg/min)		NHOH-PhIP reduction (nmol/mg/min)
	Breast	Liver	Colon	Liver
Microsomes	0.23	1.25 ± 0.08	0.38	0.92 ± 0.05
Cytosol	^*	^*	^*	NP

All reactions performed in PBS, pH 7.4, with 1 mM NADH, 3 mM ascorbate, and 100 μM substrate, with 1 mg breast or colon microsomes or cytosol, and 0.5 mg human liver microsomes or cytosol.

Not detected. NP = not performed. Data represent the mean of 2 experiments run in duplicate, except for breast and colon microsomes, one experiment, each run in duplicate.