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. Author manuscript; available in PMC: 2008 Aug 15.
Published in final edited form as: Biochemistry. 2006 Jul 4;45(26):7990–7997. doi: 10.1021/bi0603322

Figure 4.

Figure 4

The gp45 (clamp), when loaded onto single molecules of forked DNA by the clamp loader (gp44/62) bound to DNA, forms an active holoenzyme upon addition of gp43. Each frame represents the fluorescence of single molecules of DNA with proteins bound in the order indicated at the side of each row. The filter sets are as described in the legend to Figure 2. The proteins were in 20 mM Tris, 5 mM magnesium acetate, 1 mM dithiothreitol, pH 7.9. For the addition of gp45 and gp44/62, 2.5 mM ATP was included. The loss of fluorescence upon the addition of dNTPs indicates the holoenzyme maintains strand displacement activity on single DNA molecules.