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. Author manuscript; available in PMC: 2008 Aug 15.
Published in final edited form as: Biochemistry. 2006 Jul 4;45(26):7990–7997. doi: 10.1021/bi0603322

Figure 7.

Figure 7

Plot of the fraction of bound A488-gp43 molecules with a lifetime τ, Nτ/NTotal, versus τ. Nτ is the number of molecules with a lifetime τ, and NTotal is the total number of molecules undergoing exchange, 38. The concentration of unlabelled gp43 in 20 mM Tris, 5 mM magnesium acetate, 1 mM dithiothreitol, pH 7.9 was 300 nM. After 1 hour, 20% of the bound A488-gp43 underwent exchange. In the absence of unlabeled gp43, less than 10% of the single gp43 molecules was observed to dissociate from the forked DNA in the same time period. The clamp protein (gp45) was also present on the DNA.