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. 1999 Feb;10(2):245–257. doi: 10.1091/mbc.10.2.245

Table 1.

S. pombe yeast strains and plasmids

Strain Genotype
TE119 hus1-14 ura4-294 h+
TE271 972 h−
TE366 ura4-D18 h−
TE397 ade6-M216/ade6-M210 ura4-D18/ura4-D18 leu1-32/leu1-32 h−/h+
TE785 liz1-B44 ade6-M210 leu1-32 ura4-D18
TE876 cdc20-M10 leu1-32 ura4-D18 h+
TE878 cdc22-M45 leu1-32 ura4-D18 h−
TE879 liz1Δ∷LEU2 leu1-32 ura4-D18 h−
TE911 liz1-B102 ade6-M210 leu1-32 ura4-D18
TE912 liz1Δ∷LEU2 cdc22-M45 leu1-32 ura4-D18
TE913 liz1Δ∷LEU2 cdc20-M10 leu1-32 ura4-D18
TE914 liz1Δ∷LEU2 wee1-50 leu1-32 ura4-D18
TE915 liz1Δ∷LEU2 leu1-32
TE958 liz1Δ∷LEU2 cdc25-22 leu1-32 ura4-D18
TE970 wee1-50 ura4-D18 h−
Plasmid Construct
pTE52 pliz1–4 liz1+
pTE665 pliz1Δ liz1Δ∷LEU2
pTE666 pREP42-GFPliz1C GFP-liz1 (C terminus)
pTE667 pREP42-GFPliz1 GFP-liz1+