Figure 4.
804G cells were plated into the wells of a 24-well tissue culture dish (2 × 104 cells per well) or similar wells coated with 50 μg/ml RTC, 1 μg/ml hLN5, 25 μg/ml LN1, and 25 μg/ml FN for 48 hr. As indicated, the 804G cells were maintained in medium supplemented with either 50 μg/ml IgG control antibody or 50 μg/ml antibody CM6. At 48 hr the cells were trypsinized and counted. % Proliferation indicates the increase in cell number as a percentage of that observed in the untreated control cell population. At 48 hr the control cell population expanded from 2 × 104 to 1.09 × 105 cells (100%).