Figure 5.

Effects of antibodies against α6 and β1 integrins on SCg6 cell migration along 2D rBM and laminin substrata. Cell migration assays were performed for 6 h in modified Boyden chambers coated with rBM (A), laminin (B), or fibronectin (C). SCg6 cells were then fixed and stained, and the number of cells that had migrated along the ECM was counted by microscopic inspection. Cell migration assays were performed in the absence of antibodies (control [C]), in the presence of antibodies against α1, α2, α5, α6, αv, or β1 integrin subunits, or in the presence of rat (r) or hamster (h) IgGs. Results are normalized with the number of cells that had migrated along the ECM in the absence of antibodies set to 100. Means ± SD from three independent experiments are shown. In the absence of IgGs, the number of cells migrating per square millimeter was 60.1 ± 7.55 on 2D rBM, 90.3 ± 12.25 on laminin, and 102.9 ± 13.27 on fibronectin.