Figure 7.

Effect of recombinant stromelysin-1 (SL-1) on invasion and migration of SCg6 cells. (A and B) Invasion assays were performed for 24 h in modified Boyden chambers coated with rBM gels. SCg6 cells were then fixed and stained, and the number of cells that had migrated through rBM was counted by microscopic inspection. Invasion assays were performed in the absence of antibodies (control [C]) or in the presence of antibodies against α1, α2, α6, or β1 integrin subunits with (+ SL-1, black bars) or without (− SL-1, white bars) the addition of recombinant stromelysin-1 to the culture medium. Results are normalized with the num-ber of cells migrating through rBM in the absence of antibodies and stromelysin-1 set to 100. Means ± SD from three independent experiments are shown. In the absence of antibodies, 183.2 ± 16.21 cells/mm² migrated through rBM. (C) Two-dimensional cell migration assays were performed for 6 h in modified Boyden chambers coated with rBM. SCg6 cells were then fixed and stained, and the number of cells that had migrated along rBM was counted by microscopic inspection. Cell migration assays were performed in the absence of additives (control) or in the presence of recombinant stromelysin-1, the matrix metalloproteinase inhibitor GM6001, or its inactive homologue GM1210. Results are normalized with the number of cells that had migrated along rBM in the absence of additives set to 100. Means ± SD from three independent experiments are shown. Under control conditions, 72.8 ± 9.16 cells/mm² migrated along rBM.