Figure 8.

Effect of antibodies against α1 and α2 integrins on stromelysin-1 promoter activity in SCg6 cells. Luciferase (A, C, and D) and β-galactosidase (B) activity of SCg6 cells cotransfected with SL-1 promoter–luciferase and RSV promoter–β-galactosidase constructs was analyzed 2 d after maintenance of cells on rBM gels (A and B), on tissue culture plastic (C), or in suspension culture (poly-HEMA, D). Cells were cultured in the presence of increasing concentrations of antibodies against α1 (▵), α2 (•), α6 (▪), or β1 (○) integrin subunits (A and C) or in the presence of 10 μg/ml antibodies (B and D). Results are normalized with values obtained for cells maintained in the absence of antibodies (control [C] in B and D) set to 100. Means ± SD from three independent experiments are shown. In the absence of antibodies, the average number of relative light units obtained per well was 22371 on rBM, 6580 on plastic, and 20689 on poly-HEMA for the stromelysin-1 promoter and 9618 on rBM for the RSV promoter.