Figure 3.

Mutated forms of NrfC and NapG are rapidly degraded in wild-type cells but stable in ΔtatABCDE cells. Pre-NrfC, M1 and M2 were expressed in WT cells (black squares, triangles and circles, respectively) and ΔtatABCDE cells (white squares, triangles and circles, respectively). Cells were analysed at times (in hours) after induction of synthesis with arabinose, and the graph shows the amounts of protein present (by quantitative immunoblotting) as a function of time. The immunoblots are shown in the inset, with precursor and mature forms of the protein indicated (pre, mat).