Table 1.
Plasmids and strains used in the study
| Strain/plasmid | Description | Source/reference |
|---|---|---|
| DH5α | F_ _80lacZ_M15 _(lacZYA-argF) U169 deoR recA1 endA1 hsdR17 (rk_, mk_) gal-phoA supE44 thi-1 gyrA96 relA1 | Invitrogen, Carlsbad, CA, USA |
| MC4100AR | AraR, FaraD139ΔlacU169 rpsL150 relA1 flB5301 deoC1 ptsF25 rbsR | Casadaban et al (1979); Bolhuis et al (2000) |
| ΔtatABCDE | As MC4100AR; ΔtatABCDE | Wexler et al (2000); Bolhuis et al (2000) |
| ΔtatA | As MC4100AR; ΔtatA | Sargent et al (1998); Bolhuis et al (2000) |
| ΔtatB | As MC4100AR; ΔtatB | Sargent et al (1999); Bolhuis et al (2000) |
| ΔtatC | As MC4100AR; ΔtatC | Bogsch et al (1998); Bolhuis et al (2000) |
| ΔtatE | As MC4100AR; ΔtatE | Sargent et al (1998); Bolhuis et al (2000) |
| ΔtatAE | As MC4100AR; ΔtatAE | Sargent et al (1998); Bolhuis et al (2000) |
| pJDT1 | PBAD24 expressing ssTorA–GFP under control of pBAD promoter | Thomas et al (2001) |
| pCM1 | PBAD24 expressing pre-NrfC with a hexa-Histag under control of pBAD promoter | This study |
| pCM2 | As pCM1 but point mutations C152A and C155A in the nrfC gene | This study |
| pCM3 | As pCM1 but point mutations C168A and C172A in the nrfC gene | This study |
| pCM4 | PBAD24 expressing pre-NapG with a hexa-Histag under control of pBAD promoter | This study |
| pCM5 | As pCM4 but point mutations C107A and C111A in the napG gene | This study |
| pCM6 | As pCM1 but point mutations R5K and R6K in the nrfC gene | This study |
| pCM7 | As pCM2 but point mutations R5K and R6K in the nrfC gene | This study |
| pCM8 | As pCM3 but point mutations R5K and R6K in the nrfC gene | This study |