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. 2008 Jul 17;27(15):2077–2090. doi: 10.1038/emboj.2008.134

Figure 5.

Figure 5

Recombinant Alix coated on the substratum promotes NIH/3T3 cell spreading, alignment and fibronectin matrix assembly. (A) NIH/3T3 cells were seeded onto mock-, GST- or GST–Alix-coated coverslips, and cell images were then taken under a phase-contrast microscope at low and high cell densities. (B) NIH/3T3 cells were cultured on mock-, GST- or GST–Alix-coated coverslips and cultured for 48 h, and cells were immunostained with anti-fibronectin antibodies (green) and counterstained with PI (red). (C) DOC-soluble and DOC-insoluble fractions of the proteins were extracted and immunoblotted with anti-FN antibodies. (D) Total proteins extracted from NIH/3T3 cells grown on mock-, GST- or GST–Alix-coated coverslips were immunoblotted with antibodies for each of the indicated proteins.