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. 2008 Jul 24;27(15):2043–2054. doi: 10.1038/emboj.2008.136

Figure 2.

Figure 2

Overexpression of cargo proteins increases the size and number of ERES. (A) HeLa cells were transfected with a plasmid encoding YFP-tagged wild-type GAT1. After 24 h, the cells were stained for Sec31, and images were acquired by confocal microscopy. Arrows, transfected cells; arrowheads, non-transfected cells. Graphic representation of the number (B) and intensity (C) of ERES of control (ctrl) cells, cells treated with 5 μg/ml BFA for 40 min (BFA) or cells overexpressing wild-type GAT1 (GAT-WT). (D) HeLa cells were transfected with plasmids encoding GFP-tagged VSVG-tsO45 and cultured at the permissive temperature. After 24 h, the cells were fixed, stained for Sec31, and analysed by confocal microscopy. (E, F) Graphic representation of the number (E) and intensity (F) of ERES of control (ctrl) cells or cells overexpressing VSVG-tsO45 (VSVG).