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. 2008 Jul 24;27(15):2043–2054. doi: 10.1038/emboj.2008.136

Figure 4.

Figure 4

Effect of knockdown of Sec16 on ERES. (A) HeLa cells were transfected with a control siRNA (ctrl) or two different siRNAs against Sec16 (siRNA#1, siRNA#2). After 72 h, the cells were lysed and subjected to SDS–PAGE followed by immunoblotting with antibodies against Sec16, Sec24D, and tubulin. (B) HeLa cells were transfected with a control siRNA (control) or with siRNAs against Sec16. After 72 h, the cells were fixed and immunostained for Sec31 to label ERES. (C) Number of ERES per cell and their average intensity (D). *Statistically significant differences (P⩽0.01, non-paired Student's t-test); NS, nonsignificant differences.