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Ligand binding properties of NR3B. (A) Saturation assay for [3H]glycine; the open circles indicate nonspecific binding measured in the presence of 100 mM glycine. (B) Displacement assays for D-serine, 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), and D-cycloserine (DCS). The measured Kd value for glycine is 16.4 nM; the calculated Kd values for D-serine, CNQX, and DCS are 163±45 nM, 0.93±0.09 μM, and 176±43 μM, respectively; error bars represent mean±s.e.m. from three experiments.
