Skip to main content
. 2008 Jul 24;27(15):2091–2101. doi: 10.1038/emboj.2008.141

Figure 1.

Figure 1

Epidermal inactivation of insulin receptor, IGF-1 receptor affects epidermal morphogenesis. (A) PCR analysis on genomic DNA isolated from tail biopsies showing the different genotypes of the mice: 1, K14-Cre; 2, IRfl/+;IGF-1Rfl/+; 3, IRfl/fl;IGF-1Rfl/fl; 4, K14-Cre; IRfl/fl;IGF-1R+/+; 5, K14-Cre; IR+/+;IGF-1Rfl/fl; 6,K14-Cre; IRfl/fl;IGF-1Rfl/fl. (B) PCR analysis on genomic DNA from split epidermis showing the efficiency of deletion of the floxed region in either the IR or the IGF-1R locus in the presence of keratin 14-driven Cre. (C) Western blot analysis on epidermal lysates using antibodies against either the IR receptor or the IGF-1R receptor. (D) Macroscopic appearance of control mice and mice with an epidermal deletion of IR, IGF-1R or both (dko).