Figure 5.

Expression of epidermal progenitor cell markers. (A) Western blot analysis for keratin 15 on lysates isolated from epidermis of newborn mice. Same amount of lysates was run on a separate gel and probed for actin to control for loading. (B) Real-time PCR analysis of epidermis for the indicated markers. N=5 for both control and dko. (C) Keratin 15 (green) staining on back skin of newborn mice. Nuclei were counterstained using propidium iodide (red). Scale bar is 100 μm. Inset shows high magnification of basal cell layer. (D) Quantification of K15-positive basal IFE cells and HF in a 500 μM area of control, IR^epi−/−, IGF-1R^epi−/− and dko epidermis. N=4 independent mice per genotype with 5–6 sections per mouse. (E) FACS analysis of CD34 expression on ctr, IGF-1R^−/− and dko primary keratinocytes. The average expression of four independent experiments is shown. See Supplementary Figure 4A for a representative profile.