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. 1999 Feb;10(2):283–296. doi: 10.1091/mbc.10.2.283

Figure 3.

Figure 3

Deletion of Cyk1 domains. (A) Schematic diagrams of CYK1 deletion constructs: ΔCHD (pKT 34), ΔIQ (pKT31), ΔGRD (pKT 30), ΔN (pKT33), and IQ motifs (pKT39). (B) Expression of Cyk1 deletions. Extracts were prepared after release from α-factor arrest in glucose (to shut off expression of the full-length Cyk1p expressed under the GAL1 promoter) and blotted with mouse anti-myc antibody. Lane 1, RLY 565 (full-length Cyk1-myc); lane 2, RLY 555 (ΔGRD); lane 3, RLY 556 (ΔIQ); lane 4, RLY 557 (ΔCHD). (C) Cyk1 deletions do not rescue the Cyk1− cells. (a) RLY 565, (b) RLY 555, (c) RLY 556, and (d) RLY 557 cells grown on an SC-His galactose plate (left), or on an SC-His glucose plate (right) at 30 °C for 3 d.