Table 1.
Yeast strains
| Name | Genotype | Source |
|---|---|---|
| RLY 230 | MATa ura3-52 leu2-3, 112 his3-Δ200 lys2-801 Δcyk1::LEU2 | Lippincott and Li, 1998 |
| RLY 237 | MATa ura3-52 leu2-3, 112 his3-Δ200 lys2-801 Δcyk1::LEU2 pCYK1-GFP (pRL166) | This work |
| RLY 261 | MATa ura3-52 leu2-3, 112 his3-Δ200 trp1-1 ade2 Δbar1 | Elion lab |
| RLY 277 | MATa ura3-52 leu2-3, 112 his3-Δ200 lys2-801 trp1-1 Δbar1 Δcyk1::LEU2 pGAL1-CYK1-myc (pRL170) | Lippincott and Li, 1998 |
| RLY 397 | MATa ura3-52 leu2-3, 112 his3-Δ200 trp1-1 ade2 Δbar1 pGAL1-myc-CYK1ΔIQ (pKT27) | This work |
| RLY 398 | MATa ura3-52 leu2-3, 112 his3-Δ200 trp1-1 ade2 Δbar1 pGAL1-myc-CYK1ΔGRD (pKT28) | This work |
| RLY 399 | MATa ura3-52 leu2-3, 112 his3-Δ200 trp1-1 ade2 Δbar1 pGAL1-myc-CYK1ΔCHD (pKT29) | This work |
| RLY 458 | MATa ura3-52 leu2-3, 112 his3-Δ200 trp1-1 ade2 Δbar1 pGAL1-HA-CYK1ΔN (pKT33) | This work |
| RLY 555 | MATa ura3-52 leu2-3, 112 his3-Δ200 lys2-801 trp1-1 Δbar1 Δcyk1::LEU2 pGAL1-CYK1-myc (pRL170) pCYK1ΔGRD-myc (pKT30) | This work |
| RLY 556 | MATa ura3-52 leu2-3, 112 his3-Δ200 lys2-801 trp1-1 Δbar1 Δcyk1::LEU2 pGAL1-CYK1-myc (pRL170) pCYK1ΔIQ-myc (pKT31) | This work |
| RLY 557 | MATa ura3-52 leu2-3, 112 his3-Δ200 lys2-801 trp1-1 Δbar1 Δcyk1::LEU2 pGAL1-CYK1-myc (pRL170) pCYK1ΔCHD-myc (pKT34) | This work |
| RLY558 | MATa ura3-52 leu2-3, 112 his3-Δ200 lys-2-801 trp1-1 Δbar1 Δcyk1::LEU2 pGAL1-CYK1-myc (pRL170) pMYO1-GFP (pKT36) | This work |
| RLY 559 | MATa ura3-52 leu2-3, 112 his3-Δ200 lys2-801 trp1-1 Δbar1 Δcyk1::LEU2 pGAL1-CYK1-myc (pRL170) pCYK1ΔGRD-myc (pKT30) pMYO1-GFP (pKT36) | This work |
| RLY 560 | MATa ura3-52 leu2-3, 112 his3-Δ200 lys2-801 trp1-1 Δbar1 Δcyk1::LEU2 pGAL1-CYK1-myc (pRL170) pCYK1ΔIQ-myc (pKT31) pMYO1-GFP (pKT36) | This work |
| RLY 561 | MATa ura3-52 leu2-3, 112 his3-Δ200 lys2-801 trp1-1 Δbar1 Δcyk1::LEU2 pGAL1-CYK1-myc (pRL170) pCYK1ΔCHD-myc (pKT34) pMYO1-GFP (pKT36) | This work |
| RLY 565 | MATa ura3-52 leu2-3, 112 his3-Δ200 lys2-801 trp1-1 Δbar1 Δcyk1::LEU2 pGAL1-CYK1-myc (pRL170) pCYK1-myc (pTL12) | This work |
| RLY 578 | MATa ura3-52 leu2-3, 112 his3-Δ200 trp1-1 ade2 Δbar1 pGAL1-HA-IQmotifs (pKT39) | This work |
All strains are in the W303 background, except RLY 230 and RLY 237, which are the S288C background.