Table 2.
The formation of Cyk1 (myc) or actin rings in strains bearing Cyk1 deletions
| Strain | No. of cells counted | No. of myc rings | No. of actin rings |
|---|---|---|---|
| RLY 277 (full-length) (arrested and released in galactose) | 655 | 23 | 23 |
| RLY 277 (full-length) | 580 | 0 | 0 |
| RLY 555 (ΔGRD) | 1390 | 35 | 35 |
| RLY 556 (ΔIQ) | 1375 | 0 | 0 |
| RLY 557 (ΔCHD) | 1665 | 25 | 0 |
Cells were arrested with α-factor in the presence of 2% glucose (except as noted), released from arrest, and fixed at 15-min intervals beginning when most cells were budded until cells rebudded. Because of the transient nature of the structure and because we did not want to bias toward a particular timepoint, the numbers are totals from five time points. The numbers are consistent with the observation that approximately 5% of cells in an exponentially growing population have Cyk1p rings. Cyk1p was visualized by immunofluorescence using anti-myc antibody, and actin was stained with rhodamine phallodin.