Fig. 1.

Comparison of GAPT with LAT family adaptor proteins. (A) Comparison of conserved tyrosine residues between GAPT and other LAT family members. Similar to LAT, GAPT has a short, extracellular domain, a transmembrane (TM) domain, and multiple cytoplasmic tyrosine residues. Among these tyrosine residues, four of them are within a Grb2-binding motif (YxN; gray). (B) Sequence alignment of hGAPT and mGAPT, which share 58% homology of amino acid sequences. The conserved transmembrane domain is labeled, and potential palmitoylation sites are boxed. Potential Grb2-binding motifs are underlined. Arrows show the conserved prolines. The molecular weights of hGAPT and mGAPT are estimated to be 17.8 and 17.6 kDa, respectively.