Fig. 1.

LL-37 decreases the numbers of apoptotic neutrophils. Fresh neutrophils were treated with LL-37 (0–50 μM) and stained with AV and PI and analyzed by flow cytometry (a–c) or stained with trypan blue and viable cells counted in a hemocytometer, and the results are expressed as viable cells (d) and viable cells × the percent AV⁻PI⁻ cells/(% AV⁻PI⁻ cells+% AV⁺PI⁻ cells) and reported as corrected AV⁻PI⁻ (Corr AV⁻PI⁻) cells (e) and viable cells × % AV⁺PI⁻ cells/(% AV⁻PI⁻ cells+% AV⁺PI⁻ cells) and reported as corrected AV⁺PI⁻ (Corr AV⁺PI⁻) cells (f) versus time after treatment. Black (0 μM), blue (0.5 μM), green (1.0 μM), red (5.0 μM), purple (10.0 μM), and magenta (50.0 μM); n = 4; *, P < 0.05; **, P < 0.01; ***, P < 0.001, versus untreated controls (0 μM).