Fig. 3.

Sensitization of Raji cells to sTNF-α-induced cytotoxicity by the NF-κB inhibitor PDTC. (A and B) Raji cells (5×10⁴) were treated with different concentrations of PDTC as indicated for 1 h. (C and D) Raji cells (5×10⁴) pretreated with 5 μM PDTC for 1 h were incubated with 100 U/ml sTNF-α for 24 h. Untreated cells and cells treated with sTNF-α or PDTC alone served as controls. Cell viability was measured by MTT, and NF-κB activity was determined by ELISA. Data represent the mean of three independent experiments; *, P < 0.01.