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CD1d stimulation on human primary monocytes. (A) CD1d and CD66 expression on human primary monocytes, which (B) were isolated by immunomagnetic purification. Purity and viability were evaluated by CD14/PI staining. (C) Isolated monocytes were stimulated with anti-CD1d antibody overnight, and then cell viability was determined by CD14/PI staining. All experiments were repeated at least twice, and a typical result is shown here. SSH, side scatter height; FSH, forward scatter height.
