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. Author manuscript; available in PMC: 2008 Aug 15.
Published in final edited form as: Biochemistry. 2007 Apr 12;46(18):5437–5445. doi: 10.1021/bi0616347

Figure 2.

Figure 2

Reduction of enzyme-bound FMN by NADPH under anaerobic conditions, pH 7.0, at 37°C. Shown are spectra of 25 µM FMN in assay buffer (dark blue), FMNH− formed immediately after mixing with 2 mM NADPH (red), and after the subsequent addition of 50 µM IPP (dark green).